EasyPro Human 11,12-dihydroxyeicosatrienoic acids(11,12-DHETs)ELISA Kit

Description

Human 11,12-dihydroxyeicosatrienoic acids(11,12-DHETs)ELISA Kit

Catalog NO.
FY-EH2396S

Alternative Names
11,12-DHETs

Species Reactivity
Human

Test Method
Competitive

Reaction Time
45min

Samples Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL

Preparation and Storage Store all reagents at 2-8 degree C.

Sensitivity
0.94 ng/mL

Detection range
1.57-100ng/mL

Please be aware that our products are for research use only and are not intended for diagnostic or therapeutic use

Product Information

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human 11,12-DHETs. During the reaction, Human 11,12-DHETs in the sample or standard competes with a fixed amount of Human 11,12-DHETs on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human 11,12-DHETs. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human 11,12-DHETs in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Note

Please note before ordering a product that some of the data information on our website is derived from biological databases, which may sometimes result in ambiguous or irrelevant product information. This online data sheet is for your reference only. It is the customer’s responsibility to confirm and evaluate this information prior to purchasing a kit to ensure that it meets your requirements. Our product are dynamic research tools and they are subject to updates and improvements from time to time. If the latest data for the assay is important to you, then please contact our sales team for the latest version of the data sheet or reach out to our technical support team for a pre-sales consultation before placing your order. We will confirm the latest details of the test method. We cannot guarantee that the data posted online is the most up to date manual, it is provided as an example only. Please refer to the manuals supplied with the assay kit for precise details.

What’s more, due to the limited shelf life of our products, ELISA kits are not normally stored as finished products. Each kit is assembled on site upon receiving your order and is subjected to rigorous factory quality testing. In the actual manufacturing process, the range, sensitivity and precision of the product may vary slightly from the reference data sheet on the website, so please use the product in accordance with the accompanying manual for your experiments. If there are significant changes to the data, our sales team will confirm the order with you prior to shipping.

Please be aware that our products are for research use only and are not intended for diagnostic or therapeutic use.