17-OHP(17-Hydroxyprogesterone) ELISA Kit
Catalog #FY-EU1168
Sensitivity
87.3 pg/mL
Standard Range 312.5-20000 pg/mL
Application Endocrinology;Reproductive science;Hormone metabolism;
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Description
17-OHP(17-Hydroxyprogesterone) ELISA Kit
Catalog NO.
FY-EU1168
Alternative Names
17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
Species Reactivity
Universal
Test Method
Competitive
Reaction Time
1.5H
Samples Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
Preparation and Storage Store all reagents at 2-8 degree C.
Sensitivity
87.3 pg/mL
Detection range
312.5-20000 pg/mL
Please be aware that our products are for research use only and are not intended for diagnostic or therapeutic use
Product Information
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 17-OHP. During the reaction, 17-OHP in the sample or standard competes with a fixed amount of 17-OHP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 17-OHP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 17-OHP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Note
Please note before ordering a product that some of the data information on our website is derived from biological databases, which may sometimes result in ambiguous or irrelevant product information. This online data sheet is for your reference only. It is the customer’s responsibility to confirm and evaluate this information prior to purchasing a kit to ensure that it meets your requirements. Our product are dynamic research tools and they are subject to updates and improvements from time to time. If the latest data for the assay is important to you, then please contact our sales team for the latest version of the data sheet or reach out to our technical support team for a pre-sales consultation before placing your order. We will confirm the latest details of the test method. We cannot guarantee that the data posted online is the most up to date manual, it is provided as an example only. Please refer to the manuals supplied with the assay kit for precise details.
What’s more, due to the limited shelf life of our products, ELISA kits are not normally stored as finished products. Each kit is assembled on site upon receiving your order and is subjected to rigorous factory quality testing. In the actual manufacturing process, the range, sensitivity and precision of the product may vary slightly from the reference data sheet on the website, so please use the product in accordance with the accompanying manual for your experiments. If there are significant changes to the data, our sales team will confirm the order with you prior to shipping.
Please be aware that our products are for research use only and are not intended for diagnostic or therapeutic use.
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