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Chicken IL17(Interleukin 17) ELISA Kit

Product No

FY-ECH1522

Sample Type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details
Product Name in English:
Chicken IL17(Interleukin 17) ELISA Kit
Species:
Chicken
Alternative Name:
IL-17;IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Assay Type:
Competitive
Standard:
1000 pg/mL
Sensitivity:
25.4 pg/mL
Detection Range:
15.63-1000 pg/mL
Assay Time:
1.5H
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Research:
Cytokine;Infection immunity;
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IL17 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL17. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL17 in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
1000 0.268 0.269 0.269
500 0.368 0.37 0.369
250 0.562 0.565 0.564
125 0.823 0.825 0.824
62.5 1.118 1.119 1.119
31.25 1.513 1.524 1.519
15.63 1.827 1.829 1.828
0 2.491 2.499 2.495
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-93 85
EDTA plasma (n=8) 87-99 93
Cell culture media (n=8) 90-103 96
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 96-101 87-98 95-102
Average (%) 98 95 96
1:4 Range (%) 85-92 87-98 89-97
Average (%) 86 97 94
1:8 Range (%) 80-96 92-104 86-102
Average (%) 91 98 99
1:16 Range (%) 89-101 82-96 94-106
Average (%) 99 86 101
Assay Procedures
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