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Chicken fT4(Free Thyroxine) ELISA Kit

Product No

FY-ECH1371

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details
Product Name in English:
Chicken fT4(Free Thyroxine) ELISA Kit
Species:
Chicken
Alternative Name:
FTI
Assay Type:
Competitive
Standard:
8 ng/mL
Sensitivity:
0.1 ng/mL
Detection Range:
0.13-8 ng/mL
Assay Time:
1.5H
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Research:
Signal transduction;
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with fT4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to fT4. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
8 0.195 0.196 0.196
4 0.331 0.331 0.331
2 0.546 0.55 0.548
1 0.88 0.886 0.883
0.5 1.308 1.309 1.309
0.25 1.7 1.704 1.702
0.13 2.076 2.083 2.08
0 2.516 2.531 2.524
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 89-103 96
EDTA plasma (n=8) 86-99 92
Cell culture media (n=8) 83-97 90
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 89-102 85-94 85-95
Average (%) 101 88 85
1:4 Range (%) 92-101 85-94 86-93
Average (%) 98 93 91
1:8 Range (%) 88-102 87-101 91-101
Average (%) 102 93 92
1:16 Range (%) 87-102 89-99 87-98
Average (%) 93 90 96
Assay Procedures
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