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Sheep vWF(Von Willebrand Factor) ELISA Kit

Product No

FY-ESh1269

Sample Type

Plasma.

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details
Product Name in English:
Sheep vWF(Von Willebrand Factor) ELISA Kit
Species:
Sheep
Alternative Name:
F8VWF; VWD; von Willebrand antigen 2
Assay Type:
Competitive
Standard:
800 ng/mL
Sensitivity:
4.11 ng/mL
Detection Range:
12.5-800 ng/mL
Assay Time:
1.5H
Sample Type:
Plasma.
Research:
Signal transduction;Hematology;
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with vWF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to vWF. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of vWF in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
800 0.198 0.199 0.199
400 0.355 0.355 0.355
200 0.565 0.566 0.566
100 0.829 0.831 0.83
50 1.113 1.117 1.115
25 1.413 1.423 1.418
12.5 1.747 1.749 1.748
0 2.543 2.546 2.545
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-92 86
EDTA plasma (n=8) 90-105 97
Cell culture media (n=8) 88-102 95
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 87-95 80-91 86-99
Average (%) 89 80 86
1:4 Range (%) 85-96 80-91 92-104
Average (%) 87 84 92
1:8 Range (%) 92-102 89-103 82-96
Average (%) 95 91 92
1:16 Range (%) 81-92 82-95 95-102
Average (%) 90 89 100
Assay Procedures
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