Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit

Mouse

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

P43432

Sandwich

250pg/mL

0.47 pg/mL

3.91-250pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-12 p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-12 p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-12 p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-12 p40 in the samples is then determined by comparing the OD of the samples to the standard curve.