Mouse AP-1(Transcription Factor AP-1) ELISA Kit

Mouse

v-jun avian sarcoma virus 17 oncogene homolog,jun oncogene,c-Jun,AP-1,c-jun,Activator Protein-1,p39,Proto-oncogene c-Jun,Transcription factor AP-1

P05627

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.