Mouse S1PR2(Sphingosine 1 Phosphate Receptor 2) ELISA Kit

Mouse

Sphingosine 1-phosphate receptor 2,S1PR2,S1P receptor 2,S1P2,Endothelial differentiation G-protein coupled receptor 5,Sphingosine 1-phosphate receptor Edg-5,S1P receptor Edg-5,EDG5

P52592

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Endocrinology;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1PR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1PR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S1PR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1PR2 in the samples is then determined by comparing the OD of the samples to the standard curve.