Mouse SDF-1β(Stromal Cell Derived Factor 1 Beta) ELISA Kit

Mouse

Stromal cell-derived factor 1,CXCL12,SDF-1,hSDF-1,C-X-C motif chemokine 12,Intercrine reduced in hepatomas, IRH,hIRH,Pre-B cell growth-stimulating factor,PBSF,SDF1, SDF1A, SDF1B

P40224

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

CD & Adhesion molecule;Apoptosis;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDF-1β. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SDF-1β. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SDF-1β, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SDF-1β in the samples is then determined by comparing the OD of the samples to the standard curve.