Mouse RAPGEF2(Rap Guanine Nucleotide Exchange Factor 2) ELISA Kit

Mouse

RAPGEF2, CNrasGEF, NRAPGEP, PDZ-GEF1, PDZGEF1, RA-GEF, RA-GEF-1, Rap-GEP, nRap GEP, Rap guanine nucleotide exchange factor 2

Q8CHG7

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Tumor immunity;Infection immunity;Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAPGEF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RAPGEF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAPGEF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAPGEF2 in the samples is then determined by comparing the OD of the samples to the standard curve.