Mouse HIF-2α(Hypoxia Inducible Factor 2 Alpha) ELISA Kit

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Product No

FY-EM4032

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples

Assay Time

3.5H

Price($)

280 400

Size

48T 96T

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Product Details

Product Name in English:

Mouse HIF-2α(Hypoxia Inducible Factor 2 Alpha) ELISA Kit

Species:

Mouse

Alternative Name:

EPAS1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, endothelial PAS domain protein 1, Hypoxia-inducible factor-2alpha

Uniport ID:

P97481

Assay Type:

Sandwich

Standard:

20ng/mL

Sensitivity:

0.19 ng/mL

Detection Range:

0.32-20ng/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples

Research:

Endocrinology;Cardiovascular biology;Hepatology;Hormone metabolism;

Test Principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF-2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HIF-2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF-2α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HIF-2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
20	2.353	2.369	2.361	2.284
10	1.559	1.561	1.56	1.49
5	0.928	0.932	0.93	0.859
2.5	0.445	0.445	0.445	0.376
1.25	0.266	0.268	0.267	0.197
0.63	0.166	0.167	0.167	0.097
0.32	0.119	0.119	0.119	0.05
0	0.069	0.069	0.069	0

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	82-95	88
EDTA plasma (n=8)	83-98	91
Cell culture media (n=8)	91-99	95

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-92	95-103	80-94
1:2	Average (%)	91	100	85
1:4	Range (%)	80-96	86-92	87-96
1:4	Average (%)	94	92	88
1:8	Range (%)	93-105	91-102	85-98
1:8	Average (%)	103	93	98
1:16	Range (%)	90-99	89-97	95-104
1:16	Average (%)	93	90	101

Assay Procedures

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