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Human AP13(Apelin 13) ELISA Kit

Product No

FY-EH4745

Sample Type

serum, plasma, Tissue homogenate and Other biological samples

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details
Product Name in English:
Human AP13(Apelin 13) ELISA Kit
Species:
Human
Alternative Name:
AP13
Uniport ID:
Q9ULZ1(65~77aa)
Assay Type:
Competitive
Standard:
8000pg/mL
Sensitivity:
75 pg/mL
Detection Range:
125-8000pg/mL
Assay Time:
1.5H
Sample Type:
serum, plasma, Tissue homogenate and Other biological samples
Research:
Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
8000 0.204 0.205 0.205
4000 0.361 0.362 0.362
2000 0.571 0.572 0.572
1000 0.835 0.836 0.836
500 1.119 1.124 1.122
250 1.419 1.426 1.423
125 1.753 1.763 1.758
0 2.549 2.573 2.561
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 88-102 95
EDTA plasma (n=8) 80-97 88
Cell culture media (n=8) 83-95 89
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 86-95 80-91 91-97
Average (%) 88 80 95
1:4 Range (%) 82-93 80-91 95-104
Average (%) 89 87 101
1:8 Range (%) 97-106 86-92 97-105
Average (%) 103 90 104
1:16 Range (%) 89-102 89-96 82-95
Average (%) 100 90 86
Assay Procedures
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