Human RANκL(Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit

Human

TNFSF11, TRANCE, RANKL, CD254, ODF, OPGL, OPTB2, hRANKL2, sOdf

O14788

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

saliva, Serum, Cell culture supernatant

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RANκL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RANκL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RANκL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RANκL in the samples is then determined by comparing the OD of the samples to the standard curve.