Human ICAM-3/CD50(Intercellular Adhesion Molecule 3) ELISA Kit

Human

Cluster of Differentiation 5,ICAM3,CDW5,ICAM-R,CD_antigen: CD5

P32942

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Tumor immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICAM-3/CD50. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ICAM-3/CD50. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ICAM-3/CD50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ICAM-3/CD50 in the samples is then determined by comparing the OD of the samples to the standard curve.