Human PD-L1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit

Human

CD274, B7-H1, PD-L1, PDCD1L1, PDL1, B7 homolog 1

Q9NZQ7

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Infection immunity;Endocrinology;Bone metabolism;Rheumatology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PD-L1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PD-L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PD-L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PD-L1 in the samples is then determined by comparing the OD of the samples to the standard curve.