Human SP-B(Pulmonary surfactant-associated protein B) ELISA Kit

Human

SFTPB, PSP-B, SFTB3, SFTP3, SMDP1, surfactant protein B,18 kDa pulmonary-surfactant protein,6 kDa protein,Pulmonary surfactant-associated proteolipid SPL(Phe)

P07988

Sandwich

50ng/mL

0.47 ng/mL

0.79-50ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Tumor immunity;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SP-B in the samples is then determined by comparing the OD of the samples to the standard curve.