Human LMP7/PSMB8(Low molecular mass protein 7/Proteasome subunit beta type-8) ELISA Kit

Human

PSMB8, ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PSMB5i, RING1, proteasome subunit beta 8

Sandwich

500ng/mL

4.69 ng/mL

7.82-500ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LMP7/PSMB8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LMP7/PSMB8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LMP7/PSMB8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LMP7/PSMB8 in the samples is then determined by comparing the OD of the samples to the standard curve.