Human LCA/CD45(Leukocyte Common Antigen) ELISA Kit

Human

PTPRC, B22, CD45, CD45R, GP18, L-CA, LCA, LY5, T2, protein tyrosine phosphatase, receptor type C

P08575

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LCA/CD45. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LCA/CD45. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LCA/CD45, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LCA/CD45 in the samples is then determined by comparing the OD of the samples to the standard curve.