Human TU3A(TU3A Protein) ELISA Kit

Human

Down regulated in renal cell carcinoma 1,Down-regulated in renal cell carcinoma 1,Downregulated in renal cell carcinoma,DRR1,F17A,FAM17A,Family with sequence similarity 17, member A,FLJ3158,FLJ45473,Protein FAM17A,Protein TU3A,TU3A

O95990

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TU3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TU3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TU3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TU3A in the samples is then determined by comparing the OD of the samples to the standard curve.