Human CD3e(Cluster of Differentiation 3e) ELISA Kit

Human

T-cell surface glycoprotein CD3 epsilon chain,CD3e molecule, epsilon,CD3E,IMD18,T3E,TCRE,CD3e molecule,T-cell surface antigen T3/Leu-4 epsilon chain,CD_antigen: CD3e

P07766

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD3e. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD3e. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD3e, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD3e in the samples is then determined by comparing the OD of the samples to the standard curve.