Human CDH19(Cadherin 19) ELISA Kit

Human

DCHS1, CDH19, CDH25, CDHR6, FIB1, PCDH16, VMLDS1, MVP2, dachsous cadherin-related 1, Protein dachsous homolog 1, protocadherin-16, cadherin-19, cadherin-25, fibroblast cadherin-1, KIAA1773

Q96JQ0

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Human gene deletion;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDH19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CDH19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDH19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CDH19 in the samples is then determined by comparing the OD of the samples to the standard curve.