Human Ki67P(Ki-67 Protein) ELISA Kit

Human

Antigen KI-67,Ki-67,MKI67,marker of proliferation Ki-67, KIA, MIB-, MIB-1, PPP1R15,Proliferation marker protein Ki-67,Antigen identified by monoclonal antibody Ki-67,Antigen Ki67

P46013

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki67P. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ki67P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki67P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ki67P in the samples is then determined by comparing the OD of the samples to the standard curve.