Human DBT(Dihydrolipoamide branched chain transacylase E2) ELISA Kit

Human

DBT, BCATE2, BCKAD-E2, BCKADE2, E2, E2B, Lipoamide acyltransferase component of branched- chain alpha-keto acid dehydrogenase complex, mitochondrial

P11182

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

CD & Adhesion molecule;Apoptosis;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DBT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DBT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DBT in the samples is then determined by comparing the OD of the samples to the standard curve.