Human RNLS(Renalase) ELISA Kit

Human

65344N21Rik,AI452315,AW644,C1orf59,Chromosome 1 open reading frame 59,FLJ11218,HGNC:25641,Hypothetical protein LOC55328,MAO C,MAO-C,mMAO C,Monoamine oxidase C,Monoamine oxidase-C,Renalase,Renalase FAD dependent amine oxidase,RNLS

Q5VYX0

Sandwich

500ng/mL

4.69 ng/mL

7.82-500ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNLS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RNLS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNLS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNLS in the samples is then determined by comparing the OD of the samples to the standard curve.