Human FCRL3(Fc receptor-like protein 3) ELISA Kit

Human

Fc receptor-like protein 3,FCRL3, FcR-like protein 3,FcRL3,Fc receptor homolog 3,FcRH3,IFGP family protein 3,hIFGP3,Immune receptor translocation-associated protein 3,SH2 domain-containing phosphatase anchor protein 2,CD37c,IFGP3,IRTA3,SPAP2

Q96P31

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FCRL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FCRL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FCRL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FCRL3 in the samples is then determined by comparing the OD of the samples to the standard curve.