Human THRα(Thyroid Hormone Receptor Alpha) ELISA Kit

Human

THRA, AR7, CHNG6, EAR7, ERB-T-1, ERBA, ERBA1, NR1A1, THRA1, THRA2, c-ERBA-1, nuclear receptor subfamily 1, group A, member 1

P10827

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Tissue homogenate

Metabolic pathway;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to THRα. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THRα. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain THRα, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THRα in the samples is then determined by comparing the OD of the samples to the standard curve.