Human IL-33(Interleukin 33) ELISA Kit

Human

IL33, C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, Interleukin 33, IL-33

O95760

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Endocrinology;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-33. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-33. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-33 in the samples is then determined by comparing the OD of the samples to the standard curve.