Human CK-19/KRT19(Cytokeratin 19) ELISA Kit

Human

CK19, K19, Keratin 19, K1CS, Keratin, type I cytoskeletal 19, 4-kDa Keratin Intermediate Filament

P08727

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;CD & Adhesion molecule;Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CK-19/KRT19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CK-19/KRT19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CK-19/KRT19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CK-19/KRT19 in the samples is then determined by comparing the OD of the samples to the standard curve.