Human SAP25(Synaptosome Associated Protein 25) ELISA Kit

Human

SNAP25, CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ168F16.2, SUP, synaptosome associated protein 25kDa, synaptosome associated protein 25

P60880

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAP25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SAP25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SAP25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SAP25 in the samples is then determined by comparing the OD of the samples to the standard curve.