Human HSP gp96(Heat Shock Protein glycoprotein 96) ELISA Kit

Human

Heat shock protein 9kDa beta member 1 ,HSP9B1, endoplasmin,gp96,grp94, ERp99

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP gp96. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HSP gp96. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP gp96, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP gp96 in the samples is then determined by comparing the OD of the samples to the standard curve.