Human GRIN2B(Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B) ELISA Kit

Human

GRIN2B, GluN2B, MRD6, NMDAR2B, NR2B, hNR3,Glutamate [NMDA] receptor subunit epsilon-2,N-methyl D-aspartate receptor subtype 2B

Q13224

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Tissue homogenate and Cell lysate

Metabolic pathway;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GRIN2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GRIN2B in the samples is then determined by comparing the OD of the samples to the standard curve.