Human GPRC5A(G Protein Coupled Receptor, Family C, Group 5, Member A) ELISA Kit

Human

Retinoic acid-induced protein 3,GPCR5A, RAI3, RAIG1, PEIG-1, TIG1, G protein-coupled receptor class C group 5 member A,Phorbol ester induced gene 1,Retinoic acid-induced gene 1 protein,RAIG-1

Q8NFJ5

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Tumor immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPRC5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GPRC5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPRC5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GPRC5A in the samples is then determined by comparing the OD of the samples to the standard curve.