Human α2-AP(α2-Antiplasmin) ELISA Kit

Human

Alpha 2-antiplasmin,α2-antiplasmin,plasmin inhibitor,Alpha-2-plasmin inhibitor,Alpha-2-PI,SERPINF2, A2AP, AAP, ALPHA-2-PI, API, PLI

P08697

Sandwich

800ng/mL

7.5 ng/mL

12.5-800ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to α2-AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to α2-AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain α2-AP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of α2-AP in the samples is then determined by comparing the OD of the samples to the standard curve.