Human MMP-12(Matrix Metalloproteinase 12) ELISA Kit

Human

MMP12, HME, ME, MME, Macrophage Metalloelastase, Macrophage Elastase

P39900

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Tumor immunity;Hematology;Developmental science;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MMP-12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP-12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP-12 in the samples is then determined by comparing the OD of the samples to the standard curve.