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Biotin ELISA Kit

Product No

FY-EU1028

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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DataSheet DataSheet 说明书 说明书 MSDS MSDS
Product Details
Product Name in English:
Biotin ELISA Kit
Species:
Universal
Alternative Name:
Biotin
Assay Type:
Competitive
Standard:
200 pg/mL
Sensitivity:
4.7 pg/mL
Detection Range:
3.13-200 pg/mL
Assay Time:
1.5H
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Research:
Enzyme & Kinase;
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Biotin protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biotin. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
200 0.178 0.179 0.179
100 0.314 0.317 0.316
50 0.529 0.534 0.532
25 0.863 0.867 0.865
12.5 1.291 1.292 1.292
6.25 1.683 1.695 1.689
3.13 2.059 2.06 2.06
0 2.499 2.499 2.499
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 93-107 100
EDTA plasma (n=8) 87-99 93
Cell culture media (n=8) 87-95 91
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 83-98 89-97 85-99
Average (%) 92 92 91
1:4 Range (%) 88-97 89-97 80-93
Average (%) 92 90 81
1:8 Range (%) 96-105 85-94 89-97
Average (%) 102 93 95
1:16 Range (%) 86-92 80-96 85-94
Average (%) 89 92 88
Assay Procedures
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