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EasyPro Universal NE(Noradrenaline) ELISA Kit
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Product No

FY-EU10132S

Sample Type

Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Time

45min

Price($)

300 420

Size

48T 96T

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Product Details
Product Name in English:
EasyPro Universal NE(Noradrenaline) ELISA Kit
Species:
Universal
Alternative Name:
NA; NAd; Norepinephrine; Norepi
Assay Type:
Competitive
Standard:
5000pg/mL
Sensitivity:
26.1 pg/mL
Detection Range:
78.13-5000 pg/mL
Assay Time:
45min
Sample Type:
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Research:
Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NE Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for NE are added to the micro ELISA plate wells. NE in samples (or standards) competes with a fixed amount of NE on the solid phase supporter for sites on the HRP linked detection antibody specific to NE. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of NE in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
5000 0.188 0.189 0.189
2500 0.326 0.331 0.329
1250 0.545 0.552 0.549
625 0.876 0.892 0.884
312.5 1.297 1.301 1.299
156.25 1.754 1.759 1.757
78.13 2.063 2.075 2.069
0 2.543 2.566 2.555
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-104 85-97 92-105
Average (%) 97 90 95
1:4 Range (%) 86-102 85-97 85-97
Average (%) 95 86 89
1:8 Range (%) 80-93 87-96 90-99
Average (%) 85 89 94
1:16 Range (%) 89-97 82-95 82-101
Average (%) 96 86 97
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 92-106 99
EDTA plasma (n=8) 80-95 86
Cell culture media (n=8) 88-103 95
Assay Procedures

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