EasyPro Human Copeptin(CPP)ELISA Kit

Human

CT-proAVP, C-Terminal Provasopressin, Vasopressin-Neurophysin 2-Copeptin

P01185(126~164aa)

Competitive

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Enzyme & Kinase;Infection immunity;Endocrinology;Bone metabolism;Rheumatology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CPP Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for CPP are added to the micro ELISA plate wells. CPP in samples (or standards) competes with a fixed amount of CPP on the solid phase supporter for sites on the HRP linked detection antibody specific to CPP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of CPP in the samples is then determined by comparing the OD of the samples to the standard curve.