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EasyPro Rat LEP(Leptin) ELISA Kit
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Product No

FY-ER2739S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

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Product Details Performance Procedures Citations
Product Details
Product Name in English:
EasyPro Rat LEP(Leptin) ELISA Kit
Species:
Rat
Alternative Name:
LEPD, OB, OBS, Obesity Homolog
Uniport ID:
P50596
Assay Type:
Sandwich
Standard:
10ng/mL
Sensitivity:
0.1 ng/mL
Detection Range:
0.16-10ng/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL
Research:
Signal transduction;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LEP. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for LEP are added to the micro ELISA plate wells. LEP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to LEP. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of LEP. You can calculate the concentration of LEP in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
10 2.391 2.397 2.394 2.361
5 1.706 1.714 1.71 1.677
2.5 0.99 1.006 0.998 0.965
1.25 0.483 0.49 0.487 0.454
0.63 0.244 0.247 0.246 0.213
0.32 0.121 0.123 0.122 0.089
0.16 0.074 0.075 0.075 0.042
0 0.033 0.033 0.033 0
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 95-107 95-102 82-93
Average (%) 103 96 91
1:4 Range (%) 92-103 95-102 89-103
Average (%) 94 98 97
1:8 Range (%) 81-99 85-96 80-92
Average (%) 91 90 84
1:16 Range (%) 90-98 90-98 88-97
Average (%) 96 98 94
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 87-99 93
EDTA plasma (n=8) 92-105 98
Cell culture media (n=8) 83-95 89
Assay Procedures

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