EasyPro Rat Pulmonary surfactant-associated protein D(SP-D)ELISA Kit

Rat

SFTPD, COLEC7, PSP-D, SFTP4, SP-D, surfactant protein D, Lung surfactant protein D, Collectin-7, Pulmonary surfactant-associated protein D

P35248

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Signal transduction;Tumor immunity;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-D. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for SP-D are added to the micro ELISA plate wells. SP-D in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to SP-D. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SP-D. You can calculate the concentration of SP-D in the samples by comparing the OD of the samples to the standard curve.