EasyPro Mouse Collagen Type Ⅱ(COL2)ELISA Kit

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EasyPro Series ELISA Kits

Product No

FY-EM3230S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

Purchase details

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DataSheet

说明书 MSDS

MSDS

Product Details

Product Name in English:

EasyPro Mouse Collagen Type Ⅱ(COL2)ELISA Kit

Species:

Mouse

Uniport ID:

P28481

Assay Type:

Sandwich

Standard:

4000pg/mL

Sensitivity:

37.5 pg/mL

Detection Range:

62.5-4000pg/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Research:

Enzyme & Kinase;Metabolic pathway;Hepatology;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to COL2. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for COL2 are added to the micro ELISA plate wells. COL2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to COL2. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of COL2. You can calculate the concentration of COL2 in the samples by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
4000	2.376	2.413	2.395	2.352
2000	1.536	1.565	1.551	1.508
1000	0.862	0.872	0.867	0.824
500	0.462	0.468	0.465	0.422
250	0.242	0.245	0.244	0.201
125	0.139	0.139	0.139	0.096
62.5	0.092	0.092	0.092	0.049
0	0.042	0.043	0.043	0

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	96-107	101
EDTA plasma (n=8)	90-105	97
Cell culture media (n=8)	85-97	91

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	80-96	86-97	87-98
1:2	Average (%)	87	95	95
1:4	Range (%)	86-92	86-97	92-101
1:4	Average (%)	89	93	100
1:8	Range (%)	96-105	88-95	82-98
1:8	Average (%)	100	91	86
1:16	Range (%)	89-103	92-101	89-97
1:16	Average (%)	102	95	93

Assay Procedures

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