EasyPro Series ELISA Kits

EasyPro Mouse C5a(Complement Component 5a) ELISA Kit
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Product No

FY-EM1098S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL

Assay Time

1.5H

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Product Details
Product Name in English:
EasyPro Mouse C5a(Complement Component 5a) ELISA Kit
Species:
Mouse
Alternative Name:
C5a
Uniport ID:
P06684(679~1680aa)
Assay Type:
Sandwich
Standard:
4000pg/mL
Sensitivity:
37.5 pg/mL
Detection Range:
62.5-4000pg/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
Research:
Cytokine;Infection immunity;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C5a. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for C5a are added to the micro ELISA plate wells. C5a in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to C5a. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of C5a. You can calculate the concentration of C5a in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
4000 2.358 2.379 2.369 2.345
2000 1.518 1.522 1.52 1.496
1000 0.844 0.855 0.85 0.826
500 0.444 0.447 0.446 0.422
250 0.224 0.225 0.225 0.201
125 0.121 0.121 0.121 0.097
62.5 0.074 0.074 0.074 0.05
0 0.024 0.024 0.024 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-93 86
EDTA plasma (n=8) 90-105 97
Cell culture media (n=8) 86-99 92
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-96 86-97 83-96
Average (%) 91 89 84
1:4 Range (%) 86-95 83-96 92-104
Average (%) 94 96 103
1:8 Range (%) 90-103 91-103 89-101
Average (%) 102 93 97
1:16 Range (%) 87-90 96-105 85-97
Average (%) 89 103 88
Assay Procedures
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