EasyPro Human Pulmonary surfactant-associated protein A1(SP-A1)ELISA Kit

Human

MGC133365, PSAP, PSPA, SFTP1, SFTPA1

Q8IWL2

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

1.5H

Serum, Tissue homogenate, Cell lysate, Cell culture supernatant, Alveolar lavage fluid(Serum, tissuehomogenates, celllysates, cellculturesupernates, lunglavagefluid)；Sample Volume=50uL

Enzyme & Kinase;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-A1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for SP-A1 are added to the micro ELISA plate wells. SP-A1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to SP-A1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SP-A1. You can calculate the concentration of SP-A1 in the samples by comparing the OD of the samples to the standard curve.