EasyPro Human Pulmonary surfactant-associated protein B(SP-B)ELISA Kit

Human

SFTPB, PSP-B, SFTB3, SFTP3, SMDP1, surfactant protein B,18 kDa pulmonary-surfactant protein,6 kDa protein,Pulmonary surfactant-associated proteolipid SPL(Phe)

P07988

Sandwich

50ng/mL

0.47 ng/mL

0.79-50ng/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Cytokine;Tumor immunity;Infection immunity;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-B. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for SP-B are added to the micro ELISA plate wells. SP-B in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to SP-B. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SP-B. You can calculate the concentration of SP-B in the samples by comparing the OD of the samples to the standard curve.