EasyPro Human Insulin(INS)ELISA Kit

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EasyPro Series ELISA Kits

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Product No

FY-EH3153S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

Purchase details

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DataSheet

说明书 MSDS

MSDS

Product Details

Product Name in English:

EasyPro Human Insulin(INS)ELISA Kit

Species:

Human

Alternative Name:

IDDM2;ILPR;IRDN;MODY1

Uniport ID:

P01308

Assay Type:

Sandwich

Standard:

50μIU/mL

Sensitivity:

0.47 μIU/mL

Detection Range:

0.79-50μIU/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Research:

Enzyme & Kinase;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to INS. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for INS are added to the micro ELISA plate wells. INS in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to INS. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of INS. You can calculate the concentration of INS in the samples by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
50	2.504	2.516	2.51	2.44
25	1.648	1.652	1.65	1.58
12.5	0.982	0.989	0.986	0.916
6.25	0.463	0.469	0.466	0.396
3.13	0.254	0.257	0.256	0.186
1.57	0.156	0.157	0.157	0.087
0.79	0.104	0.105	0.105	0.035
0	0.069	0.07	0.07	0

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	91-99	95
EDTA plasma (n=8)	82-95	88
Cell culture media (n=8)	83-98	91

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	86-97	87-98	89-100
1:2	Average (%)	89	93	91
1:4	Range (%)	90-98	87-98	80-91
1:4	Average (%)	92	90	84
1:8	Range (%)	80-93	85-92	89-103
1:8	Average (%)	88	88	100
1:16	Range (%)	92-105	80-96	82-95
1:16	Average (%)	96	80	86

Assay Procedures

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