EasyPro Human Brain Natriuretic Peptide(BNP)ELISA Kit

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EasyPro Series ELISA Kits

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Product No

FY-EH3193S

Sample Type

Plasma；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

Purchase details

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DataSheet

说明书 MSDS

MSDS

Product Details

Product Name in English:

EasyPro Human Brain Natriuretic Peptide(BNP)ELISA Kit

Species:

Human

Alternative Name:

NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B

Uniport ID:

P16860

Assay Type:

Sandwich

Standard:

8000pg/mL

Sensitivity:

75 pg/mL

Detection Range:

125-8000pg/mL

Assay Time:

1.5H

Sample Type:

Plasma；Sample Volume=50uL

Research:

Metabolic pathway;Infection immunity;Hematology;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BNP. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for BNP are added to the micro ELISA plate wells. BNP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to BNP. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BNP. You can calculate the concentration of BNP in the samples by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
8000	2.44	2.461	2.451	2.429
4000	1.589	1.594	1.592	1.57
2000	0.947	0.952	0.95	0.928
1000	0.439	0.442	0.441	0.419
500	0.212	0.216	0.214	0.192
250	0.102	0.102	0.102	0.08
125	0.057	0.057	0.057	0.035
0	0.022	0.022	0.022	0

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	87-99	93
EDTA plasma (n=8)	93-107	100
Cell culture media (n=8)	87-95	91

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	88-97	95-104	89-103
1:2	Average (%)	90	101	93
1:4	Range (%)	86-92	95-104	85-102
1:4	Average (%)	87	103	91
1:8	Range (%)	80-103	87-96	81-96
1:8	Average (%)	90	95	82
1:16	Range (%)	89-97	82-96	87-105
1:16	Average (%)	94	95	98

Assay Procedures

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