EasyPro Series ELISA Kits

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EasyPro Human Hornerin (HRNR)ELISA Kit

Product No

FY-EH3647S

Sample Type

serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Time

45min

Price($)

300 420

Size

48T 96T

Purchase details

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MSDS MSDS
Product Details
Product Name in English:
EasyPro Human Hornerin (HRNR)ELISA Kit
Species:
Human
Assay Type:
Sandwich
Standard:
5000pg/mL
Sensitivity:
46.88 pg/mL
Detection Range:
78-5,000pg/mL
Assay Time:
45min
Sample Type:
serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HRNR Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for HRNR are added to the micro ELISA plate wells. HRNR in samples (or standards) competes with a fixed amount of HRNR on the solid phase supporter for sites on the HRP linked detection antibody specific to HRNR. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of HRNR in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
5000 2.458 2.489 2.474 2.451
2500 1.602 1.616 1.609 1.586
1250 0.936 0.955 0.946 0.923
625 0.417 0.425 0.421 0.398
312.5 0.208 0.21 0.209 0.186
156.25 0.11 0.111 0.111 0.088
78.13 0.058 0.058 0.058 0.035
0 0.023 0.023 0.023 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 84-93 87
EDTA plasma (n=8) 95-108 101
Cell culture media (n=8) 82-97 89
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 80-102 88-103 95-102
Average (%) 82 90 100
1:4 Range (%) 83-96 88-103 80-91
Average (%) 89 101 80
1:8 Range (%) 80-93 86-92 87-96
Average (%) 89 89 89
1:16 Range (%) 88-97 91-99 83-101
Average (%) 97 92 93
Assay Procedures
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