EasyPro Series ELISA Kits

EasyPro Universal Lipoxin A4(LXA4)ELISA Kit

Product No

FY-EU10151S

Sample Type

serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Time

45min

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Product Details
Product Name in English:
EasyPro Universal Lipoxin A4(LXA4)ELISA Kit
Species:
Universal
Alternative Name:
LX-A4
Assay Type:
Competitive
Standard:
40000pg/mL
Sensitivity:
175.4 pg/mL
Detection Range:
625-40000 pg/mL
Assay Time:
45min
Sample Type:
serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Research:
Signal transduction;Metabolic pathway;Infection immunity;
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LXA4 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for LXA4 are added to the micro ELISA plate wells. LXA4 in samples (or standards) competes with a fixed amount of LXA4 on the solid phase supporter for sites on the HRP linked detection antibody specific to LXA4. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of LXA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
40000 0.186 0.189 0.188
20000 0.322 0.322 0.322
10000 0.537 0.547 0.542
5000 0.871 0.879 0.875
2500 1.299 1.309 1.304
1250 1.691 1.701 1.696
625 2.067 2.1 2.084
0 2.507 2.511 2.509
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 83-99 95
EDTA plasma (n=8) 85-97 91
Cell culture media (n=8) 85-97 91
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-97 95-104 89-103
Average (%) 90 101 93
1:4 Range (%) 86-92 95-104 85-102
Average (%) 87 103 91
1:8 Range (%) 80-103 87-96 81-96
Average (%) 90 95 82
1:16 Range (%) 89-97 82-96 87-105
Average (%) 94 95 98
Assay Procedures
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