EasyPro Universal 25-Hydroxyvitamin D3(HVD3)ELISA Kit

Universal

25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

Competitive

200ng/mL

0.91 ng/mL

3.13-200 ng/mL

45min

serum, plasma and other biological fluids

Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HVD3 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for HVD3 are added to the micro ELISA plate wells. HVD3 in samples (or standards) competes with a fixed amount of HVD3 on the solid phase supporter for sites on the HRP linked detection antibody specific to HVD3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.