EasyPro Universal 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a)ELISA Kit

Universal

8-EpiPGF2-α; 8epi-PGF2a

Competitive

2000pg/mL

9.55 pg/mL

31.25-2000 pg/mL

45min

serum, plasma and other biological fluids

Endocrinology;Pulmonology;Hepatology;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2a Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for 8-epi-PGF2a are added to the micro ELISA plate wells. 8-epi-PGF2a in samples (or standards) competes with a fixed amount of 8-epi-PGF2a on the solid phase supporter for sites on the HRP linked detection antibody specific to 8-epi-PGF2a. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.