EasyPro Universal Vitamin B2(VB2)ELISA Kit

Universal

Riboflavin; Riboflavine

Competitive

600ng/mL

2.55 ng/mL

9.38-600 ng/mL

45min

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Nutrition metabolism;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VB2 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for VB2 are added to the micro ELISA plate wells. VB2 in samples (or standards) competes with a fixed amount of VB2 on the solid phase supporter for sites on the HRP linked detection antibody specific to VB2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of VB2 in the samples is then determined by comparing the OD of the samples to the standard curve.